A NOVEL APPROACH TO REDUCE OXIDATIVE STRESS AT MOLECULAR AND CELLULAR LEVEL WITH APPLICATIONS IN REGENERATIVE MEDICINE



PROJECT NO:139/2011

PROJECT CODE:PN-II-ID-PCE-2011-3-0769

TITLE: “A NOVEL APPROACH TO REDUCE OXIDATIVE STRESS AT MOLECULAR AND CELLULAR LEVEL WITH APPLICATIONS IN REGENERATIVE MEDICINE”

PROJECT DIRECTOR:       Prof. Dr IRINEL POPESCU

INSTITUTUL CLINIC FUNDENI BUCURESTI

DATE OF BEGINNING THE PROJECT: 05/10/2011

DATE OF FINALISING THE PROJECT: 05/12/2015

TOTAL VALUE OF THE PROJECT: 1.495.000 ron

FINNANCING AUTHORITY: UEFISCDI


PROJECT OBJECTIVE:

 

The main objective of the proposed project is to investigate the  anti-oxidant , protective  ( and others) effects of High Density Green Photons (HDGP) on biological systems and the possibles optical manipulations  at different molecular and cellular levels

 

ABSTRACT:


The interactions between electromagnetic field and biological systems were extensivelyinvestigated, with remarkable results and advanced technologies. Nevertheless, the visible domain of the spectrum has been rather neglected, since classic physics did not allow electronic transitions induced by visible light.

Starting with the ’70 decade a research group centered around the Society of Quantum Biology (San Francisco) advanced the idea of possible “molecular activations”, different from the “electronic excitations” level. Phenomenological models have been published, based on the molecular coherence at macroscopic scale with the ability of maintaining long-lived coherence, (see the pioneering paper of Fröhlich  on long range coherence in the enzyme activity). Within this group, Comorosan  published the first experimental evidence for these possible “molecular activations” performing experiments on enzymes and revealing kinetics changes through low level irradiations of their substrates. A string of papers on this new phenomenology appeared in the international journals of that time period . The field expanded with the advent of new theoretical developments and of new type of technologies, in areas of free-space molecular diffraction, magnetic biomolecules, quantum optics and Josephson devices . A large new type of evidence appeared from the nanotechnology and from the biological field. Molecular films were designed displaying a surprising molecular coherence with space and time stability. The modern domain of quantum computing provided a wealth of data on biological quantum coherence and molecular superposition states. An important contribution has been put forward by Penrose  in connection with molecular coherence in biological structures.

Penrose considers quantum coherence the circumstances when large numbers of particles can collectively cooperate in a single quantum state which remains essentially unentangled with its environment. This large scale quantum coherence is found in Bose-Einstein condensation (which occurs in laser action) where the entire system behaves as a whole, and in superconductors, when electrons have a way of “pairing up” in Cooper pairs, which behave as individual bosons.

Theoretical and experimental developments by the Comorosan group  renewed the interes tof visible light interaction with biological structuresThe interaction between electromagnetic field and matter was long and extensively studied both in the country and also abroad.

Recently, the interaction of light with matter generated a new scientific domain known in physics as optical manipulation with the new concepts of optical matter and optical force.

 

PERSONNEL LIST:


 

  1. 1. Prof Dr Irinel  Popescu MD PhD    Senior researcher Project Director
  2. Prof Dr  Doc Comorosan Sorin Nicolae Titus,  Senior reseacher Member of Romanian Academy
  3. Prof Dr LilianaLiviaPaslaru,  PhD   Senior researcher
  4. Dr. SimonaOlimpiaDima,  MD, PhD  Senior researcher
  5. Dr Razvan Iacob, MD PhD    Senior researcher
  6. Prof Dr  Ioan  Stamatin, PhD Senior reseacher
  7. Ionescu Elena, technician
  8. Anca  Mihaela Nastase, PhD student
  9. Dr Radu Mitrica MD PhD student

10.  Laura Stefan  Biologist

 

PHASES:

Phase 1 (15/12/2011)– FINISHED

Phase 2 (15/12/2012)-FINISHED

Phase 3 (16/12/2013)FINISHED

Phase 4 (15/12/2014)-FINISHED

Phase 5 (05/10/2015)-FINISHED

Phase 6 (05.10.2016)- FINISHED


PUBLICATION LIST:



 
1-    Green light effects on biological systems: a new biophysical phenomenon,  S. Comorosan, S. Polosan, S. Jipa, I. Popescu, G. Marton, E. Ionescu, L. Cristache, D. Badila, R. Mitrica, J. Photochem. Photobiol. B. 102 (2011) 39.
 
2-    High-density green photons effects on NaCl solutions detected by red blood cells membranes, R. Mitrica, I. Popescu, L. Paslaru, D. Badila, S. Polosan, L. Cristache, E. Ionescu, C. Tataru, S. Comorosan, Digest Journal of Nanomaterials and Biostructures Vol. 7, No. 1, January – March 2012, p. 227 – 235
 
3-    Antioxidant effects induced in biological macromolecular systems by high density photons through localized excitations, S. Comorosan, S. Polosan, I.Popescu, L. Paslaru, A. Nastase, R. Mitrica, E. Ionescu, Biochimica et Biophysica Acta (BBA)- Bioenergetics, 1817, 1, 2012.
 
4-    Localized excitations induced in nonlinear complex biological systems by high density green photons, S. Comorosan, I. Popescu, S. Polosan, I. Farcasanu, L. Paslaru, E. Ionescu, L. Cristian Cune, M. Apostol, in Springer Verlag, Localized excitations in non-linear systems series, New York, vol.3 (2013).
 
5-    Optical manipulation of complex molecular systems: experimental and theoretical evidence, S. Comorosan, I. Popescu, S. Polosan, I. Stamatin, E. Ionescu, S. Avramescu, L. C. Cune, M. Apostol, Eur. Phys. J. B (2013) 86: 232.
 
6-    Ileana C. Farcasanu, Radu Mitrica, Ligia Cristache, Ioana Dumitru, Lavinia L. Ruta, Liliana Paslaru, Sorin Comorosan, “Optical manipulation of Saccharomyces cerevisiae cells reveals that green light protection against UV irradiation is favored by low Ca2+ and requires intact UPR pathway FEBS Letters 2013 nov 1,587(21):3514-21

 

7-    Sorin Comorosan, Silviu Polosan, Irinel Popescu, Ioan Stamatin, Elena Ionescu, Sorin Avramescu, Liviu Cristian Cune, Marian Apostol. Optical manipulation of complex molecular systems by high density green photons: experimental and theoretical evidence. The European Physical Journal B, 2013; 86 (5)
 
8-     L Paslaru,  A Nastase ,  L Stefan, R Florea, A Sorop, E Ionescu,  I Popescu, S.Comorasan . Stimulatory and possible antioxidant effects of high density green photons(HDGP) on cellular systems Journal of Medicine and Life, vol7,issue4,oct-dec 2014,pp 619-622

 

9-     Sorin Comorosan,  Irinel Popescu, Silviu Polosan, Cristian Parvu, Elena Ionescu, Liliana Paslaru, Marian Apostol  “. Conformational changes and metastable states induced in proteins by green light. European Physical Journal B (2015) 88: 8.
 
10-  Petruta Alexandru, Liliana Paslaru ,Simona Dima,Anca Nastase, Laura Stoichita  S.Comorosan and  I. Popescu  “Functional characterization of 1.1B4  novel human insulin releasing  cell line and effect of HDGP (High Density Green Photons) irradiation on beta pancreatic cells and human pancreatic islets” Journal of Translational Medicine and Research vol 21,Nr 3,sept 2016,183-192
 
11- Sorin Comorosan, Ileana Farcasanu, Silviu Polosan, Sorin Avramescu, Marian Apostol, Elena Ionescu, Liliana Paslaru and Irinel Popescu  “Protective Effect of Green Light Against the Deleterious Effects of UV Irradiation on Cellular Systems” Journal of Translational Medicine and Research vol 21,Nr 3,sept 2016,193-200
 


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Expected Results

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Expected Results